This study determines the occurrence and concentration levels of artificial low calorie sweeteners (LCS) in food and food supplements on the Italian market. The analysed sample set (290 samples) was representative for the Italian market and comprised of beverages; jams; ketchups; confectionery; dairy products; table-top sweeteners and food supplements. All samples were analysed via UPLC-MS/MS. The method was in-house validated for the analysis of seven LCS (aspartame; acesulfame-K; saccharin; sucralose; cyclamate; neotame and neohesperidin dihydrochalcone in food and for five LCS (aspartame; acesulfame-K; saccharin; cyclamate and sucralose) in food supplements. Except for cyclamate in one beverage which exceeded the maximum level (ML) with 13%; all concentrations measured in food were around or below the ML. In food supplements 40 of the 52 samples (77%) were found to be above the ML with exceedances of up to 200% of the ML.
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